Gene Editing Analysis: Now Faster than Ever
INDUCE-seq® is a scalable, PCR-free platform for rapidly mapping both on-target and off-target DNA breaks induced during genome editing. Delivering data in as little as 2 days from sample processing, this cell-based assay offers single-nucleotide resolution, capturing each DNA break with a single sequencing read. Designed for high sensitivity, reproducibility, and genome-wide, unbiased detection, INDUCE-seq® is compatible with any gene editing system and adaptable to all cell types, including primary cells, iPSCs, and immortalized cells from diverse tissues.
- On- and off- target assessment
- Editing kinetic analysis
- Guide screening
- Nuclease development
- Editing strategy optimizations
The INDUCE-seq® On-Demand Solution
INDUCE-seq®, rapid, high-resolution detection of double-stranded breaks (DSBs) is now available as an in-lab solution, giving developers maximum control over timelines and experimental design. INDUCE-seq® On-Demand empowers developers to perform library preparation and sequencing in their own labs, then gain access to Broken String Biosciences analysis pipeline to customize data reports.
Why CGT Developers Choose INDUCE-seq®
INDUCE-seq® delivers the industry’s most comprehensive and accurate on- and off-target characterization service, enabling pharmaceutical companies to make confident go/no-go decisions early in discovery while minimizing regulatory risk throughout development.
- Comprehensive detection: Quantifies on/off target DSBs across the genome
- Cost Savings: Reduce R&D timelines and risk of regulatory holds due to off-target effects
- Cell-based assay: Compatible with all relevant cell types, including primary cells, iPSCs, T-cells, and immortalized cells from diverse tissues
- Single-nucleotide resolution: Precise DSB detection at the single nucleotide level.
- PCR-free workflow: Directly measure DSBs without amplification bias, 1 read = 1 break.
- Fast turnaround: Sample to insights in days.
- Universal compatibility: Technology is compatible with any gene editing system.
- Automation capability: Integrate with industry-standard automation systems.
INDUCE-seq® is also available through a full partnership service model. The INDUCE-seq® services offer the same accuracy and efficiencies in identifying on- and off-target gene editing, while allowing for higher levels of customization of analysis parameters. Learn more about INDUCE-seq® services here.