Gene Editing Analysis
INDUCE-seq® is a scalable, PCR-free platform simultaneous detection of both on-target and off-target DNA breaks induced during genome editing. Delivering data in as little as 2 days from sample submission, this cell-based assay offers single-nucleotide resolution, capturing each DNA break with a single sequencing read. Designed for high sensitivity, reproducibility, and genome-wide, unbiased detection, INDUCE-seq® is compatible with any gene editing system and adaptable to all cell types, including primary cells, iPSCs, and immortalized cells from diverse tissues.
- On- and off-target assessment
- Editing kinetic analysis
- Guide screening
- Nuclease development
- Editing strategy optimizations
Full-Service Gene Editing Safety Assessments
Induce-seq® is available through a full-service partnership with Broken String Biosciences, delivering expert off-target analysis to support the safety and efficacy of gene and gene-edited cell therapies. This highly scalable and customizable service enables rapid, high-resolution mapping of DNA breaks, ensuring confident decision-making during IND-enabling studies and program scale-up.
Why CGT Developers Choose INDUCE-seq®
INDUCE-seq® delivers the industry’s most comprehensive and accurate on- and off-target characterization service, enabling pharmaceutical companies to make confident go/no-go decisions early in discovery while minimizing regulatory risk throughout development.
- Comprehensive detection: Quantifies on/off target DSBs across the genome.
- Cost Savings: Reduce R&D timelines and risk of regulatory holds due to off-target effects.
- Cell-based assay: Compatible with all relevant cell types, including primary cells, iPSCs, T-cells, and immortalized cells from diverse tissues.
- Single-nucleotide resolution: Precise DSB detection at the single nucleotide level.
- PCR-free workflow: Directly measure DSBs without amplification bias, 1 read = 1 break.
- Fast turnaround: Sample to insights in days.
- Universal compatibility: Technology is compatible with any gene editing system.
- Automation capability: Integrate with industry-standard automation systems.
- Customizable: Collaboratively define the analysis parameters that suit any application.
INDUCE-seq® On-Demand is now available for clients to bring the power of gene editing analysis into their own labs. INDUCE-seq® On-Demand offers the same high accuracy and efficiency in detecting on-and off-target effects while providing maximum control over timelines, IP and experimental design. Learn more about INDUCE-seq® On-Demand here.